TopoFlow protein conformational ensemble prediction server

TopoFlow is designed for protein conformational ensemble prediction using an evolutionarily conditioned flow-matching framework. Starting from an amino-acid sequence, TopoFlow incorporates evolutionary information to guide continuous structural transformations and sample alternative protein conformations. Instead of representing a protein with only one static structure, the server focuses on generating a collection of structurally diverse states that can reflect protein flexibility and conformational heterogeneity. For each submitted target, users can paste a protein sequence or upload a single-sequence FASTA file and specify the desired number of conformations. The server standardizes the sequence input, records the sampling parameters, and places the task into the TopoFlow processing queue. After computation is complete, the generated conformations are organized on a dedicated result page and provided as a downloadable ensemble archive. TopoFlow is intended to support research on protein dynamics, alternative structural states, and structure-function relationships.